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. 2022 Aug 9;14(16):3253. doi: 10.3390/nu14163253

Figure 3.

Figure 3

Effect of increasing concentrations of KYN from 0.1 to 10 µM on fibrosis markers in TGF-β-stimulated colonic fibroblasts CCD-18Co. CCD-18Co cells were simultaneously stimulated with TGF-β (10 ng/mL) and KYN (0; 0.1; 1; 10 µM) for 24 h. α-SMA protein level (A) was studied by Western Blot and GAGDH expression serves as the loading control for the amount of protein. mRNA levels of CTGF (B), COL1A1 (C), COL3A1 (D), AhR (E) and ARNT (F) were studied by RT-qPCR. Results are expressed as mean ± SEM of four independent experiments.