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. 2022 Aug 9;14(16):3253. doi: 10.3390/nu14163253

Figure 4.

Figure 4

Effect of increasing concentration of ITE from 1 to 100 µM on fibrosis markers in TGF-β-stimulated colonic fibroblasts CCD-18Co. CCD-18Co cells were simultaneously stimulated with TGF-β (10 ng/mL) and ITE (0; 1; 10; 100 µM) for 24 h. α-SMA protein level (A) was studied by Western Blot and GAPDH expression serves as the loading control for the amount of protein. mRNA levels of CTGF (B), COL1A1 (C), COL3A1 (D), AhR (E) and ATNT (F) were studied by RT-qPCR. Results are expressed as mean ± SEM of four independent experiments.