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. 2022 Aug 4;14(8):1720. doi: 10.3390/v14081720

Figure 1.

Figure 1

Redistribution of the nucleolar proteins UBF, Fibrillarin and Nucleophosmin 1 (NPM1) upon lytic reactivation of KSHV in iSLK-infected cells. BAC16-mCherry-ORF45-infected iSLK cells were treated for 48 h with 1 µg/mL Dox and 1 mM n-Butyrate to induce viral lytic reactivation. Uninduced infected iSLK cells were used as controls. Cells were stained with antibodies to UBF or (A), Fibrillarin followed by anti-rabbit Alexa Fluor 647-conjugated secondary antibody (B) or NPM1 followed by anti-mouse Alexa Fluor 647-conjugated antibodies (C). The corresponding staining of nuclear DNA by Hoechst is also shown. mCherry-ORF45 expression and localization and the appearance of condensed chromatin staining marked different stages of cells undergoing viral lytic reactivation. Arrows indicate different patterns of redistribution. Right-hand columns show the merged output of the nucleolar proteins immunofluorescence and Hoechst staining 150 cells were scored according to the cellular distribution of UBF along with ORF45 expression and localization and chromatin organization (D).