Figure 2.

UBF does not colocalize with PML bodies. BAC16-infected iSLK cells were treated for 48 h with 1 µg/mL Dox and 1 mM n-Butyrate to induce lytic reactivation. Uninduced BAC16-infected iSLK cells were used as a control. Cells were stained with anti-PML followed by Rhodamine-conjugated secondary antibody. Subsequently, the cells were incubated with antibodies to UBF, and then with anti-rabbit Cy5 secondary antibody. Chromatin was detected by Hoechst staining.