Figure 4.

UBF partially colocalizes with viral replication compartments whereas Fibrillarin does not colocalize with replication compartments during lytic reactivation. BAC16-infected iSLK cells were treated for 48 h with 1µg/mL Dox and 1 mM n-Butyrate to induce lytic reactivation. Untreated infected iSLK cells were used as control. Cells were stained with anti-ORF59 and secondary Rhodamine-conjugated antibody. Cells were subsequently incubated with anti-UBF (A) or anti-Fibrillarin (B) and then incubated with anti-rabbit Cy5-conjugated secondary antibody. Chromatin was detected by Hoechst staining.