Figure 5.

UBF shares common redistribution kinetics and pattern with RPA195 and PICT-1 but not with Fibrillarin during lytic reactivation. BAC16-infected iSLK cells were treated for 48 h with 1µg/mL Dox and 1 mM n-Butyrate to induce lytic reactivation. Uninduced infected iSLK cells were used as controls. Cells were stained with mouse anti-UBF and Rhodamine-conjugated secondary antibody. Cells were then incubated with anti-Fibrillarin, which was detected by anti-rabbit Cy5-conjugated secondary antibody (A). Cells were stained with rabbit anti-UBF and Cy3-conjugated secondary antibody, and then with anti-RPA194 (B) or anti-PICT-1 (C), which were detected by anti-mouse Cy5-conjugated secondary antibody. Chromatin was detected by Hoechst staining.