Figure 6.

Lytic reactivation does not alter expression levels of the 47S pre-rRNA. Uninfected SLK and BAC16-mCherry-ORF45-infected iSLK cells were treated for 48 h with 1 µg/mL Dox and 1 mM n-Butyrate to induce lytic reactivation. Untreated cells were used as controls. Lytic reactivation was confirmed by expression of mCherry-ORF45 in ~80% of cells. The 47S pre-rRNA levels were determined using RT-qPCR with primers that target the 5′ external transcribed spacer (ETS) of pre-rRNA, and the results obtained were normalized to 18S rRNA. Data are presented relative to untreated SLK cells, and are from the averages of five biological repeats, each including three technical replicates. Error bars represent standard deviations. No statistically significant differences between SLK and iSLK cells prior or after lytic reactivation were obtained (one-way ANOVA and one-sample t-test).