Figure 7.

Northern blot analysis of rRNA during viral lytic reactivation. Total RNA was extracted from uninfected SLK and BAC16-mCherry-ORF45-infected iSLK cells that were either left untreated or treated with Dox and n-Butyrate for 48 h. Lytic reactivation was confirmed by the expression of mCherry-ORF45 in ~70% of cells. A total of 20 µg RNA from each sample was resolved on denaturing 1.2% agarose gel, transferred to nylon membranes, and hybridized with probes representing 5.8S, 18S and 28S rRNA. No accumulation of pre-rRNA processing products was evident upon lytic reactivation.