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RNA in situ hybridization analysis to determine the location of the pre-rRNA transcripts and Fibrillarin in cells during viral lytic reactivation. SLK and BAC16-infected iSLK cells were treated with 1µg/mL Dox and 1 mM n-Butyrate. Cells were incubated with ITS-1 probe and stained with anti-Fibrillarin and Cy3-conjugated secondary antibody. Hoechst staining was used to detect chromatin.
