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. 2022 Aug 20;14(16):3434. doi: 10.3390/nu14163434

Figure 1.

Figure 1

Incubation with bergamot PF extract reduces intracellular lipid content in 2D cultured hepatocytes. Rat hepatoma cell line McA-Rh-7777 was cultured in 2D and incubated with 50 µM of oleic acid and different concentrations of bergamot PF extract (0.001, 0.01, 0.11 µg/mL) in regular medium without FBS for 24 h. Intracellular lipid content was measured by Oil red-O staining (A). ORO area quantified by Image J (B) and showed a significant decrease in intrahepatic lipid content. Data are shown as mean ± SD of three independent experiments. Statistical analysis methods are as follows: Student`s t-test vs. 0 * p < 0.05; ** p < 0.001. Linear regression p = 0.001. Abbreviations are as follows: PF, polyphenols fraction; FBS, fetal bovine serum; OA, oleic acid; 2D, two dimensional; SD, standard deviation; ORO, Oil red-O.