Skip to main content
. 2022 Aug 20;14(16):3434. doi: 10.3390/nu14163434

Figure 5.

Figure 5

Treatment with 1 µg/mL bergamot PF extract reduces intracellular lipid content in human liver organoid. (A) Human liver organoid was cultured as 3D spheroids for a total of 7 days. Initially, 48 h after seeding, the media was replaced every 48 h. Objective 20X. (B) Human liver organoid was cultured as 3D spheroids for a total of 7 days. Initially, 48 h after seeding, the media was supplemented with 1 µg/mL bergamot PF extract for an additional 5 days with media replacement every 48 h. Objective 20X. (D) The average volume was calculated by measuring their long and short diameter using ZEN 2.3 Lite software (Zeiss). (E) For both spheroids, cellular ATP levels remained stable between the experimental groups. (C) Intracellular lipid content measured by Oil red-O staining showed a significant reduction after incubation with bergamot PF extract. Data are shown as mean ± SD of three independent experiments. The p values were calculated by using a Mann–Whitney non-parametric test. Abbreviations are as follows: PF, polyphenols fraction; 3D, three dimensional; SD, standard deviation.