Table 1.
Primer sets and probes for DNA and gene expression detection.
| Gene | Sequences | Amplicon Length | Accession Number |
|---|---|---|---|
| EcPV2-L1 | F-5′-TTGTCCAGGAGAGGGGTTAG-3′ R-5′-TGCCTTCCTTTTCTTGGTGG-3′ |
80 | NC_012123.1:5673-7172 |
| p-EcPV2-L1 | FAM-CGTCCAGCACCTTCGACCACCA-TAMRA | ||
| EcPV2-E6 | F-5′-CGTTGGCCTTCTTTGCATCT-3′ R-5′-AGGTTCAGGTCTGCTGTGTT-3′ |
80 | NC_012123.1:5-622 |
| p-EcPV2-E6 | FAM- CCGTGTGGCTATGCTGATGACATTTGG-TAMRA | ||
| EcPV2-E7 | F-5′-CTCTGAGCAGCATCACCCTT-3′ R-5′- TCTTCCTCGTCTTCTGTGTCC-3′ |
70 | NC_012123.1:624-959 |
| p-EcPV2-E7 | FAM- AGAGCGCTCCCCCTCAGTCA-TAMRA | ||
| EcPV2-E2 | F-5′-AAAAGGGAGGGTACGTTGTC-3′ R-5′-CCTGGTAGTAGACATGCTGC-3′ |
90 | NC_012123.1:2767-4017 |
| p-EcPV2-E2 | FAM- GCCAAGACAGCCACGACGCCAT-TAMRA | ||
| B2M | F-5′-GGCTACTCTCCCTGACTGG-3′ R-5′- TCAATCTCAGGCGGATGGAA-3′ |
135 | NM_001082502.3 |
| p-B2M | FAM-ACTCACGTCACCCAGCAGAGA-TAMRA |