Table 1.
Primer sequences for real time reverse transcription polymerase chain reaction used in this study.
| Abbrev. | Gene Name | Transcript Accession Number (Ensembl) | Forward Primer (5′→3′) | Reverse Primer (5′→3′) | Size (bp) | E (%) |
|---|---|---|---|---|---|---|
| Specific genes of follicle functionality | ||||||
| AHR | Aryl Hydrocarbon Receptor | ENSOART00020003479.1 | TGGGGCTGTTTCAATGTACC | TACAGGAATCCACCGGATGT | 233 | 81.8 |
| BMP15 | Bone morphogenetic protein 15 | ENSOART00020018955.1 | TCTATTGCCCACCTGCCTGAG | TGAAGCTGATGGCGGTAAACC | 326 | 89.2 |
| CYP19A1 | Cytochrome P450 Family 19 Subfamily A Member 1 | ENSOART00020040485.1 | GGTCATCCTGGTCACCCTTCTG | GCCGGTCGCTGGTCTCGTCTGG | 119 | 100 |
| ESR1 | Estrogen receptor 1 | ENSOART00020034283.1 | CCAGTTCCTCCTCCTCCTCT | GGCTCTGATTCACGTCTTCC | 158 | 87.2 |
| ESR2 | Estrogen receptor 2 | ENSOART00020022015.1 | ACTATGGAGTCTGGTCAT | GTCGGTTCTTATCTATGGTA | 114 | 97.3 |
| FSHR | Follicle-stimulating hormone receptor | ENSOART00000004728. | GGGCCAAGTCAACTTACCACT | TGCAAATTGGATGAAGGTCA | 144 | 88.5 |
| HSD3B1 | Hydroxy-Delta-5-Steroid Dehydrogenase | ENSOART00020002039.1 | TCATTGACGTCAGGAATGCT | CTCTATGGTGCTGGTGTGGA | 128 | 84 |
| Specific genes of redox status | ||||||
| CAT | Catalase | ENSOART00020018520.1 | GAAACGCCTGTGTGAGAACA | AGCTTTCTCCCTTGCAGACA | 208 | 91.2 |
| COX4I1 | Cytochrome C Oxidase Subunit 4I1 | ENSOART00020014820.1 | AGAGCTTTGCCGAGATGAAC | TCATGTCGAGCATCCTCTTG | 182 | 88.3 |
| COX5B | Cytochrome C Oxidase Subunit 5B | ENSOART00000014875.1 | GGGCTAGAGAGGGAGGTCAT | CAGCCAGAACCAGATGACAG | 180 | 91.2 |
| GPX3 | Glutathione Peroxidase 3 | ENSOART00020022210.1 | GATGTGAACGGGGAGAAAGA | CCCACCAGGAACTTCTCAAA | 152 | 90.4 |
| GPX8 | Glutathione Peroxidase 8 | ENSOART00020019722.1 | AAGGCATTTGCAGTCTTGCT | GACCTTCAGGGTTGACCAGA | 101 | 85.3 |
| NDUFB4 | NADH Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 4 | ENSOART00020003605.1 | GGCCAGCCTACCTACTACCC | TGCATAGGTCCAACGAATCA | 181 | 90.7 |
| NDUFB5 | NADH Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 5 | ENSOART00020014020.1 | GATTGCCCGAACTTTCTTTG | AGTGCCTTATCGATGGTTGG | 174 | 82.1 |
| NDUFV2 | NADH Ubiquinone Oxidoreductase Core Subunit V2 | ENSOART00020029984.1 | TCGAAAGCCTGTTGGAAAGT | ACACCAAACCCAGGTCCTTT | 205 | 60.8 |
| NDUFAF2 | NADH Ubiquinone Oxidoreductase Complex Assembly Factor 2 | ENSOART00020010561.1 | AACAGAATGGGAAGCTTGGA | AGAGGCGTGCCCTTTAATCT | 196 | 84.2 |
| SDHA | Succinate Dehydrogenase Complex Flavoprotein Subunit A | ENSOART00000016992.1 | AGCAGAAGAAGCCGTTTGAG | TCGGTCTCGTTCAAAGTCCT | 121 | 93.3 |
| SOD1 | Superoxide Dismutase 1 | ENSOART00020002019.1 | CAAAAATGGTGTTGCCATTG | CCAGCGTTTCCAGTCTTTGT | 153 | 94.0 |
| SOD2 | Superoxide Dismutase 2 | ENSOART00020009379.1 | GGTTGGCTTGGCTTCAATAA | ACATTCCAAATGGCCTTCAG | 178 | 90.6 |
| Housekeeping genes | ||||||
| ACTB | Beta Actin | ENSOART00020013384.1 | CCAGCACGATGAAGATCAAG | ACATCTGCTGGAAGGTGGAC | 102 | 97.2 |
| RPL19 | Ribosomal Protein L19 | ENSOART00020024842.1 | CACAAGCTGAAGGCAGACAA | TGATGATTTCCTCCTTCTTGG | 129 | 95.3 |
Abbrev.: gene name abbreviation; bp: base pair; E: efficiency.