Skip to main content
. 2022 Aug 12;14(16):3314. doi: 10.3390/nu14163314

Figure 8.

Figure 8

Effect of ANA-12 on the L7Gn-induced upregulation of BDNF, p-TrkB, p-CREB, and p-ERK in the hippocampus of sleep-deprived mice. L7Gn was dissolved in 70% PEG-saline, and ANA-12 was dissolved in 1% DMSO. Mice were administered vehicle (70% PEG), L7Gn (3 mg/kg) or ANA-12 (0.5 mg/kg) once daily for five days. Western blotting and quantification of expression levels of (A) BDNF, (B) p-TrkB, (C) p-ERK, and (D) p-CREB. The expression level of BDNF was normalized against GAPDH and those of p-TrkB, p-ERK, and p-CREB were normalized against their unphosphorylated form. (A) BDNF: (one-way ANOVA: F (3, 12) = 31.64, p < 0.0001) (B) p-TrkB: (one-way ANOVA: F (3, 12) = 12.3, p = 0.0006) (C) p-ERK: (one-way ANOVA: F (3, 11) = 10.97, p = 0.0012) (D) p-CREB: (one-way ANOVA: F (3, 12) = 12.11, p = 0.0006). Data are presented as mean ± SEM (n = 3–4/group). * p < 0.05, significantly different from the control group. # p < 0.05, significantly different from the vehicle group. $ p < 0.05, significantly different from the L7Gn (3 mg/kg) group. CTL, control; Veh, vehicle.