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Scheme of thiotepa activation and drug reaction with GSH [89]. Possible GSH S-conjugates: 1: monoglutathionyl thiotepa, 2: diglutathionyl thiotepa, 3: 2-aminoethyl glutathione. P450 = cytochrome P450. Predicted bioactive sites of GSH conjugation for anticancer drug were obtained by XenoSite Reactivity Predictor available at https://swami.wustl.edu/xenosite/p/reactivity (accessed on 18 May 2022).
