FIG. 5.
Starvation survival of W3110, JP231, and their recombinant strains harboring phbCAB genes from A. eutrophus. Strains were grown in shaking flasks containing M9 minimal medium supplemented with 0.2 g of glucose liter−1. After growth was arrested, incubation was continued for 9 days under the same condition. Viable cells were counted as colonies in LB plates after appropriate dilutions. One hundred percent viability corresponds to the number of viable cells counted 1 h after entering starvation. Symbols: ○, W3110; ●, W3110(pKTPHB); □, JP231; ■, JP231(pKTPHB).