TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Relevant characteristics | Source (reference) |
|---|---|---|
| E. coli strains | ||
| W3110 | F− IN(rrnD-rrnE)1 | B. Bachmann |
| RR1 | supE44 hsdS20(rB− mB−) ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | Laboratory collection |
| CP993 | OW1 Φ(pta-1::Tn10-lacZ) | C. Park (35) |
| JP202 | RR1 Φ(pta-1::Tn10-lacZ) | This work |
| JP231 | W3110 Φ(pta-1::Tn10-lacZ) | This work |
| Plasmids | ||
| pSK2665 | phbCAB genes from A. eutrophus | A. Steinbüchel (34) |
| pKTPHB | pKT230 with the 5.2-kb BamHI-EcoRI fragment bearing phbCAB genes from pSK2665 | This work |