FIGURE 3.

Cell Growth Assay in supplemented dialyzed FBS in HEK293, HCT116, and RKO cells. Cell growth upon the application of various amino acid substrates: Alanine (Ala): 1 mM; Ala: 1 mM + NH₄ ⁺: 0.8 mM; Asparagine (Asn): 1 mM; Aspartate (Asp): 1 mM; Asp: 1 mM + NH₄ ⁺: 0.8 mM; Glutamate (Glu): 1 mM; Glu: 1 mM + NH₄ ⁺: 0.8 mM; Glutamine (Gln): 2 mM. Viable cell count was determined at every passage over the course of 31 days. Cell count data (each n = 2) were Log2-transformed and are shown as mean ± SD. The doubling time was calculated based on the duration in culture and the number of duplications underwent during this time (i.e., duration/Δlog2(cell count)).