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Isotope incorporation into glutamine after pulse labelling with 13C5-glutamate and 15N-ammonium in HCT116 and HEK293 cells. HCT116 and HEK293 cells were incubated with 13C5-glutamate and 15N-ammonium for 15 min, 30 min, 1 h, and 3 h (n = 2 each). Shown are the relative pool sizes of non-labelled (C0N0), 15N labelled (C0N1), 13C5 labelled (C5N0) 15N-13C5 labelled (C5N1) glutamine.
