(A) Emphysematous pathology was determined by digital imaging analysis of mean linear intercept measurements (Lm) of alveolar spaces at days 2, 4, and 7 after N. brasiliensis (Nb) inoculation of WT BALB/c mice.
(B–E) Il17a−/− and WT BL/6 control mice or Tcrd−/− and WT BL/6 control mice were inoculated with Nb. Then 8 days later, emphysematous pathology was assessed by Lm (B and D) and the total number of CD11b+Ly6G+ neutrophils at day 2 was determined by flow cytometry (C and E).
Each symbol represents an individual mouse, and horizontal lines indicate the mean. Data shown are the mean and SEM from at least five individual mice per group and are representative of at least two independent experiments (**p < 0.01). See also Figure S1.