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. Author manuscript; available in PMC: 2022 Aug 26.
Published in final edited form as: Cell Rep. 2018 Dec 4;25(10):2775–2783.e3. doi: 10.1016/j.celrep.2018.11.038

Figure 2. B Cell Deficiency Exacerbates Emphysematous Pathology after Nb Inoculation.

Figure 2.

(A–E) BALB/c WT and Jh−/− mice were inoculated with 600 L3 and, 7 days later, were analyzed for emphysematous pathology and IL-17 expression.

(A) Left lobe of lung was collected and photographed (scale bar, 5 mm).

(B) H&E staining of representative formalin-fixed lung sections of mice.

(C) Digital imaging analysis of mean linear intercept measurements (Lm) of alveolar spaces.

(D) Number of lung IL-17A+ γδ T cells.

(E) Number of neutrophils (CD11b+Ly6G+), analyzed by FACS.

(F–I) WT and Jh−/− mice were inoculated with 300 L3 and, 30 days later, were analyzed for emphysematous pathology. H&E staining (F) and Lm analyses (G) were assessed as described earlier, and the number of lung IL-17A+ γδ T cells (H) and lung neutrophils (CD11b+Ly6G+) (I) was determined by flow cytometry.

Each symbol represents an individual mouse, and horizontal lines indicate the mean. Data shown are representative of at least two independent experiments (*p < 0.05, **p < 0.01).