KEY RESOURCES TABLE
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
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Antibodies | ||
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Anti-Ly6G (1A8) | BD Bioscience | Cat#551461, RRID: AB_394208 |
Anti-MHCII | eBioscience | Cat# 11–5320-80, RRID: AB_2572500 |
Anti-CD11b | BD Bioscience | CaT#563553, RRID: AB_2738276 |
Anti-CD11c | BD Bioscience | Cat#564080, RRID: AB_2738580 |
Anti-Siglec F | BD Bioscience | Cat# 562681, RRID: AB_2722581 |
Anti-F4/80 | Biolegend | Cat# 123116, RRID: AB_893481 |
Anti-CD19 | BD Bioscience | Cat# 553786, RRID: AB_395050 |
Anti-B220 | Biolegend | Cat#103225, RRID: AB_389308 |
Anti-CD21 | Biolegend | Cat# 354903, RRID: AB_2561406 |
Anti-CD23 | Biolegend | Cat# 101607, RRID: AB_312832 |
Anti-CD24 | Biolegend | Cat# 101807, RRID: AB_312840 |
Anti-CD5 | Biolegend | Cat# 100610, RRID: AB_312739 |
Anti-CD1 | eBioscience | Cat# 12–0011-81, RRID: AB_465482 |
Anti-CD138 | Biolegend | Cat# 142505, RRID: AB_10960141 |
Anti-Relma | Biorbyt | Cat# orb14229, RRID: AB_10735625 |
Anti-IL-17A | R&D systems | Cat# MAB421, RRID: AB_2125018 |
Rat IgG2a isotype control (2A3) | BioXcell | Cat# BE0089, RRID: AB_1107769 |
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Critical Commercial Assays | ||
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Serum IgG1, IgE ELISA kit | Biolegend | 432405 |
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Experimental Models: Organisms/Strains | ||
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N. brasiliensis | Our Laboratory | N/A |
WT BALB/c | Jackson Laboratory | JAX:000651 |
Il10−/− BALB/c | Taconic Bioscience | Taconic 15660 |
Il4ra−/− BALB/c | Jackson Laboratory | JAX:003514 |
Jh/ BALB/c | Taconic Bioscience | Taconic:1147 |
Retnla−/− BALB/c | Provided by Dr. Marc Rothenberg | Cincinnati Children’s hospital Medical Center |
Il17a−/− BL/6 | Provided by Dr. Karen Edelblum | Rutgers-NJMS |
Tcrd−/− BL/6 | Jackson Laboratory | JAX 002120 |
WT BL/6 | Jackson Laboratory | JAX:000664 |
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Oligonucleotides | ||
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PCR primer for Il4 | Thermo Scientific | Mm00445259_m1 |
PCR primer for Il5 | Thermo Scientific | Mm00439646_m1 |
PCR primer for Il13 | Thermo Scientific | Mm99999190_m1 |
PCR primer for Il10 | Thermo Scientific | Mm00439614_m1 |
PCR primer for TGFβ | Thermo Scientific | Mm01178820 |
PCR primer for Il35 | Thermo Scientific | Mm00469294-m1 |
PCR primer for arginase 1 | Thermo Scientific | Mm00475988_m1 |
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Software and Algorithms | ||
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Statistical software program Prism | GraphPad Software | https://www.graphpad.com/ |