FIG. 4.

Glycogen content of SMEG53 grown under conditions suppressing the temperature-sensitive phenotype. Exponentially growing cultures were diluted to an OD₆₀₀ of 0.3 in fresh medium and then shifted to 42°C. Cells were grown to mid-log phase (OD₆₀₀ = 0.75 to 0.85), and then glycogen assays performed as before. Each value represents the mean of three assays ± standard deviation. (A) Mutant cells containing pMD30, pAEB234 (garA), or pAEB235 (glgE), grown in 7H9 containing 10 μg of kanamycin per ml. (B) SMEG53 cells grown in 7H9, 7H9 containing 0.2 M NaCl (NaCl), or M9.