Figure 5.

(a) Representative pictures of uninfected and infected zebrafish larvae. Top left: Uninfected larvae. In the remaining pictures, zebrafish larvae were infected with E. coli 87 (CFU = 255) encoding fluorescent mScarlet. Subsequently, they were either treated with 17fα (c = 70 μM), L8S1 (c = 3.125 μM), or a combination of both. (b) Relative share of surviving zebrafish larvae after 24 h as determined by heartbeat depending on the treatment regime. (c) Effect of different treatments on the infection progress after 24 h as determined by quantification of red pixels per larvae (zebrafish larvae pictures can be found in Figure S14). All measurements were performed in quadruplicate (n = 4 replicates with 15 Zebrafish larvae per condition, error bars = SEM). Significance was determined by one-way ANOVA and Dunnet’s multiple-comparison test (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001).