Figure 2.

(a,b) Typical nanopore-based amperometric readouts for Mol-1 and Mol-5, using 0.5 M KCl. Red star points denote enlarged events (orange panel) in the original trace. (c) The peptide sequence of Mol-1, together with that of Mol-5, 6, 7, 8, and 9, contain at least one d-amino acid. Final concentrations of 1.5 μM for Mol-1, 7.6 μM for Mol-5, 6, 7, and 8, and 0.8 μM for Mol-9 have been reached in the experiments. The color histograms show the current drop ΔI/I₀ and the logarithm of residence time t, measured at a bias voltage of −50 mV in 0.5 M KCl, 10 mM Tris, and 1 mM EDTA at pH 8.0. Moreover, the peak values of the distributions have been normalized to one with this maximum value shown in black. More detailed histograms are delineated in Figure S7. (d) Representation of Mol-5, 6, 7, 8, and 9 containing chiral amino acid reorientation in OmpF. The orientation of each sidechain has been determined from the results of the simulated dynamics delineated in Figure S8, averaged along the channel axis from 0 to 10 Å.