TABLE 1.
Plasmids and phages used in this study
| Plasmid or phage | Descriptiona | Relevant property | Selective marker(s)b | Replicon | Reference or source |
|---|---|---|---|---|---|
| pLM2 | Encodes PRD1 receptor | Kmr | IncPα | 38 | |
| pMG59 | pMS470Δ(NdeI-HindIII) Ω(PRD1 3128–4903)c | PRD1 gene II+ | Apr | pMB1 | This study |
| pML123 | pGZ119EHΔ (EcoRI-BamHI) Ω(EcoRI-XmnI adapter, RP4 XmnI-NotI, 18841–30042) | RP4 trbB-trbM+ | Cmr | ColD | 31 |
| pMS470Δ8 | pMS119EHΔ(XbaI-PstI) Ω(pT7-7 XbaI-NdeI, R751 traC AvaI-SphI) | Apr | pMB1 | 1 | |
| pWP471 | pJF119EH Ω(RP4 NspI-HaeII, 45909–46577) | RP4 traF+ | Apr | pMB1 | 54 |
| RP4 | Encodes PRD1 receptor | Apr, Kmr, Tcr | IncPα | 19 | |
| PRD1 | Wild type | 41 | |||
| PRD1sus1 | Amber mutation in gene IX | Defective in DNA packaging | 36 | ||
| PRD1sus170 | Amber mutation in gene II | Defective in adsorption | 36 | ||
| PRD1sus539 | Amber mutation in gene II | Defective in adsorption | 45 |
a
The cloning vector is indicated, followed by the restriction enzymes used and the origin of the insert in parentheses. PRD1 and RP4 coordinates were taken from the GenBank/EMBL database (accession no. M69077 and L27758, respectively).
b
Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Tcr, tetracycline resistance.
c
The PCR primers used for amplification of gene II were OP173 (TTATTCATATGGCTAATTTCAACGTGCC) and OP174 (TTTATTAAGCTTACACCTTTGAAATAATTCCGC).