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. 1999 Nov;181(21):6689–6696. doi: 10.1128/jb.181.21.6689-6696.1999

TABLE 1.

Plasmids and phages used in this study

Plasmid or phage Descriptiona Relevant property Selective marker(s)b Replicon Reference or source
pLM2 Encodes PRD1 receptor Kmr IncPα 38
pMG59 pMS470Δ(NdeI-HindIII) Ω(PRD1 3128–4903)c PRD1 gene II+ Apr pMB1 This study
pML123 pGZ119EHΔ (EcoRI-BamHI) Ω(EcoRI-XmnI adapter, RP4 XmnI-NotI, 18841–30042) RP4 trbB-trbM+ Cmr ColD 31
pMS470Δ8 pMS119EHΔ(XbaI-PstI) Ω(pT7-7 XbaI-NdeI, R751 traC AvaI-SphI) Apr pMB1 1
pWP471 pJF119EH Ω(RP4 NspI-HaeII, 45909–46577) RP4 traF+ Apr pMB1 54
RP4 Encodes PRD1 receptor Apr, Kmr, Tcr IncPα 19
PRD1 Wild type 41
PRD1sus1 Amber mutation in gene IX Defective in DNA packaging 36
PRD1sus170 Amber mutation in gene II Defective in adsorption 36
PRD1sus539 Amber mutation in gene II Defective in adsorption 45
a

The cloning vector is indicated, followed by the restriction enzymes used and the origin of the insert in parentheses. PRD1 and RP4 coordinates were taken from the GenBank/EMBL database (accession no. M69077 and L27758, respectively). 

b

Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Tcr, tetracycline resistance. 

c

The PCR primers used for amplification of gene II were OP173 (TTATTCATATGGCTAATTTCAACGTGCC) and OP174 (TTTATTAAGCTTACACCTTTGAAATAATTCCGC).