Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Schematic illustration of the experimental method for DOE. (A) Parameters and responses in manufacturing LNPs. Both parameters and responses examined in this study were expressed in bold. (B) Method for screening of LNPs in vivo. Cellular uptake, NHC class II expression, and Nluc expression in splenic DCs, Mφ, and B cells after intravenous injection of DiO-labeled mNLuc-loaded LNPs selected by DOE were measured to identify the optimal formulation.
