Figure 3.

The antinociceptive effect of (−)-2S-LP2 was antagonized by naloxone pre-treatment. Subcutaneous administration of naloxone hydrochloride (3 mg/kg) 20 min before (−)-2S-LP2 reverted its effect in the formalin test. Results are expressed as mean ± SEM (n = 6–8 mice per group) and are represented as the percentage of inhibition of pain responses of the respective vehicle group (100%). °° p < 0.01 vs. (−)-2S-LP2, ° p < 0.05 vs. (−)-2SLP2, ** p < 0.01 vs. Vehicle, two-way ANOVA followed by Bonferroni post-hoc test.