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. 2022 Aug 10;13:971482. doi: 10.3389/fpls.2022.971482

FIGURE 2.

FIGURE 2

Definition of the dormancy stages of Zhongyou 4 flower buds and expression patterns of PpMYB52. (A) Daily maximum temperature and daily minimum temperature from October 2021 to March 2022. (B) Definition of the dormancy stages of Zhongyou 4 flower buds and expression level of PpMYB52 from 15 October 2021 to 15 March 2022. At each sampling time, 25 of the shoots were placed in tap water for 25 days. If less than 50% of the buds broke dormancy, the flower buds were considered to be in the endodormancy stage; the values are the means of 25 shoots. (C) Expression of PpMYB52 in different tissues of Zhongyou 4 peach. (D) Subcellular localization of PpMYB52. PpMYB52 was fused to YFP for transient transformation into 4-week-old tobacco (N. benthamiana) leaves. 4’,6-Diamidino-2-phenylindole (DAPI) was used as a nuclear marker. The values represent the means ± SD of three replicates, and the different letters above the bars represent significant differences; P < 0.05.