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. 2022 Aug 10;13:971482. doi: 10.3389/fpls.2022.971482

FIGURE 4.

FIGURE 4

Overexpression of PpMYB52 in tomato suppresses seed germination and reduces plant height. (A) Reverse transcription polymerase chain reaction (RT-PCR) analysis results of PpMYB52-overexpressing transgenic lines. (B) qRT-PCR analysis results of PpMYB52 transgenic tomato lines under normal conditions. SlActin was used as a reference gene. (C) Phenotypes of PpMYB52-overexpressing transgenic tomato and Micro Tom wild-type tomato. (D) Plant height of PpMYB52-overexpressing transgenic tomato and Micro Tom wild-type tomato. Each group comprised 20 plants, and the results are the averages of 20 plants. (E,F) Determination of the germination of seeds of Micro Tom wild-type tomato and PpMYB52-overexpressing transgenic tomato in MS media. (G) Flowering time of PpMYB52-overexpressing transgenic tomato and Micro Tom wild-type tomato. The time is the number of days between planting and the first flower opening. Each group comprised 20 plants, and the results are the averages of 20 plants. The values represent the means ± SD of three replicates, and the different letters above the bars represent significant differences; P < 0.05.