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Dilution factors of the primary antibodies (BD Pharmingen, CA, USA) and the incubation duration for staining with the primary antibody and their source.
| Antibody Type | Cell Types and Status | Dilution Factor of Primary Antibody | Incubation Time for Primary Antibody (Hours) |
|---|---|---|---|
| CD3 | T cells | 1:50 | 1 |
| CD4 | CD4+ (Helper) T cells | 1:50 | 24 |
| CD8 | CD8+ (Cytotoxic) T cells | 1:50 | 1 |
| CD20 | B cells | 1:200 | 1 |
| CD45RO | Memory cells | 1:100 | 1 |
| CD69 | Activated cells | 1:25 | 24 |
