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NLCs are internalized by NPC in a dose-dependent manner and are devoid of cytotoxic activity. NPCs were incubated for 24 h with NLCs at concentrations between 0 and 200 µg/mL to evaluate potential cytotoxic effects (left y-axis). In the same concentration range, DiD-labeled NLCs were used to study cellular uptake (right y-axis). NLCs concentrations are expressed as µg of MM (solid lipid) per mL. Data are the means ± SEM obtained in 3 independent experiments.
