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The serum antibody titer of Bactrian camel after immunization was determined by ELISA. CD20 extracellular polypeptide was coated in microplates. Basic serum isolated before immunization was used as negative control, PBS was used as blank control, rabbit anti-camel antibody labeled with HRP was used as a secondary antibody with the working concentration of 1:10,000, and TMB was used for color development. The plate was read at OD450 nm in a microplate reader.
