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. 2022 Aug 17;10(8):1335. doi: 10.3390/vaccines10081335

Figure 9.

Figure 9

Detection of the effect of anti-CD20/CD3 BsNb on the secretion of cytokines by PBMCs in the presence or absence of Raji cells by ELISA. Raji cells were cultured in PerkinElmer Cell Carrier for 12 h with 3 parallel wells in each group, and effector cell PBMCs with the effector: target ratio = 10:1 were added to the experimental group. PBMCs cells were cultured in PerkinElmer Cell Carrier for 12 h with three parallel wells in each group and the same volume of medium added as the control group. The final concentrations of anti-CD20/CD3 BsNb were 80 μg/mL, 40 μg/mL, 20 μg/mL, 10 μg/mL, 5 μg/mL and 0 μg/mL were added to the Wells. The level of IFN-γ was determined with the ELISA method and calculated from the standard curve (** p < 0.01).