Figure 10.

The stability of BsNb was tested by mixing the BsNb with human serum and incubated for different times, then evaluating the changes in its binding activity to CD20 by ELISA. After mixing 2 μg of anti-CD20/CD3 BsNb with human serum, the mixture was determined at different time points after incubation at 37 °C. The mixed BsNbs were used as the primary antibody, and HRP-conjugated 6 × His-tagged mouse monoclonal antibody was used as the secondary antibody to detect the binding activity to CD20 antigen.