TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Relevant characteristicsa | Reference |
|---|---|---|
| S. paucimobilis | ||
| UT26 | HCH+ Nalr | 12 |
| UT103 | HCH− Nalr ΔlinD genome::Tn5 | 26 |
| UT116 | HCH− Nalr ΔlinD ΔlinE genome::Tn5 | 26 |
| P. putida PpY101 | Met Nalr | 19 |
| E. coli | ||
| MV1190 | Δlac-proAB thi supE Δsrl-recA306::Tn10 F′ traD36 proAB lacIqZΔM15 | 39 |
| HB101 | F−hsdS recA ara proA lacY galK rpsL xyl mtl supE | 21 |
| Plasmids | ||
| pRK2013 | ColE1::RK2 Tra+ Kmr | 7 |
| pKTY320::Tn5 | Apr Cm::Tn5 (Kmr Bler Strr) | 25 |
| pKS13 | RK2 replicon, cos Mob+ Tcr | 19 |
| pUC18 | pMB9 replicon, Apr | 39 |
| pUC118 | pMB9 replicon, Apr | 39 |
| pHSG399 | pMB9 replicon, Cmr | 39 |
| pAQN | pMB9 replicon, lacIqaqn Apr | 38 |
| pKSM1920 | pKS13 with about 20 kb of UT26 DNA containing linD | This study |
| pKSM208 | pKS13 with about 20 kb of UT26 DNA containing linD | This study |
| pSS1 | pUC18 containing a 1.3-kb SacI-SacI fragment upstream of linD | This study |
| pSS2 | pUC18 containing a 7-kb SacI-SacI fragment upstream of linD | This study |
| pLE1 | pUC18 carrying a 1.3-kb blunted PstI-NaeI fragment including linE at its SmaI site. linE is the same direction as the lac promoter | This study |
| pLE11 | pHSG399 carrying a 5.5-kb PstI-PstI fragment from which NaeI-NaeI fragment is removed. linE is the same direction as the lac promoter | This study |
| pPP55 | pUC18 carrying a 5.5-kb PstI-PstI fragment including linE at its PstI site. linE is the same direction as the lac promoter. | This study |
| pMYLE2 | pAQN carrying the EcoRI-NaeI fragment of pLE11 | This study |
| pCY385 | pUC18 carrying xylE | 20 |
| pLEH162A | pUC18 containing linE mutant (H162A) | This study |
| pMYH162A | pAQN containing linE mutant (H162A) | This study |
| pLEH229A | pUC18 containing linE mutant (H229A) | This study |
| pMYH229A | pAQN containing linE mutant (H229A) | This study |
| pLEE278A | pUC18 containing linE mutant (E278A) | This study |
| pMYE278A | pAQN containing linE mutant (E278A) | This study |
a
HCH+, grown on γ-HCH. aqn, aqualysin I gene of Thermus aquaticus.