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. 2022 Jul 28;10(8):1203. doi: 10.3390/vaccines10081203

Figure 2.

Figure 2

Emergence and maintenance of 5′TD EV-B populations in organs of CVB3/28-infected mice. (A) Schematic representation of EV-B cloverleaf secondary structures with stem “a” and stem-loops “b”, “c”, and “d”. Schematic representations of deletions of 8, 36, and 50 nucleotides and its impact on cloverleaf secondary structures, loss of stem “a”, stem “a” plus stem-loop “b”, and loss of stem “a” and stem-loops “b-c”, respectively. (B,C) EV-B FL and 5′TD respective viral loads were measured using a RACE-PCR method associated with a micro-electrophoresis (Agilent®) in organs of CVB3/28-infected mice (n = 8 to 10 in B; n = 3 to 6 in) (C). Data represents mean + SEM (Mann–Whitney U test; *: p < 0.05; **: p < 0.01; ***: p < 0.001, ns: non-significant). (D) Schematic representation of full-length and 5′TD populations percentages. Data represents mean. TD: terminally deleted.