Figure 4.

In vitro growth kinetics in primary swine macrophage cell cultures for ASFV-G-∆A151R and parental ASFV-G (MOI = 0.01). Samples were taken from three independent experiments at the indicated time points and titrated. Data represent means and standard deviations of three replicas. Sensitivity using this methodology for detecting virus was ≥log10 1.8 HAD₅₀/mL. No significant differences in viral yields between viruses were observed at any time point tested, which was determined using the Holm–Sidak method (α = 0.05) without assuming a consistent standard deviation. All calculations were conducted using the software Graphpad Prism version 8.