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Reversed-phase HPLC chromatograms of the noncovalently membrane-bound hemes from laccase-negative (4B and 4VI) and laccase-positive (4VII) A. lipoferum. (A) Fully aerated exponentially grown cells. (B) Fully aerated cells, stationary phase. (C) Low-aerated exponentially grown cells. (D) Low-aerated cells, stationary phase. Bacterial cultures: 1, 4B; 2, 4VI; 3, 4VII. Positions of peaks corresponding to known (D, B, and A) and unknown (R1, R2, and R3) heme species are indicated.
