Figure 2.

GCP inhibited PRV infection. (A) PK-15 cells were treated with GCP for 1 h and then infected with PRV XJ5 (MOI = 0.1) for 1 h. Cells were washed thrice with PBS and then incubated with GCP for another 24 h. (B) CPE observed by a microscope. (C) Expression levels of gB protein and actin analyzed by Western blot. (D) The internalized virus was detected by immunofluorescence assay. (E) The viral titer in supernatants was quantified by TCID₅₀. All results were obtained from three independent experiments and were presented in means ± SD (ns, not significant; ** p < 0.01; *** p < 0.001).