Figure 5.

GCP decreased PRV binding (A) PK-15 cells were incubated with PRV XJ5 (MOI = 0.1) and GCP at 4 °C for 1 h, then cells were collected at 1 hpi or 24 hpi. (B,C) At 24 hpi, the expression levels of gB protein and actin were analyzed by Western blot and the internalized virus was detected by immunofluorescence assay. (D) At 1 hpi, the copy numbers of PRV DNA were quantified by qRT-PCR. All results were obtained from three independent experiments and were presented in means ± SD (*** p < 0.001).