Figure 2.

Characterization of exosomes from plasma of healthy donors and plasma of AMI patients before and after coronary angioplasty in presence of citicoline: (a) Western-blot analysis of tetraspanins CD9, CD81, and Hsp70. (b) Acetylcholine esterase activity. Results shown are the mean ± SE of at least 8 independent exosome samples from each group. (c) Quantification of particles number with size between 50 and 100 nm by nanoparticle tracking analysis (NTA), results are the mean ± SE of at least 5 independent exosomes’ samples from each group). (d) Representative image of particles size distribution between 50 and 600 nm determined by NTA. (e) Representative transmission electron microscopy images of exosome samples of the different groups.