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. 2022 Aug 21;13(10):3151–3159. doi: 10.7150/jca.75597

Figure 2.

Figure 2

Lycorine inhibited phosphorylation p65 and IκB degradation. (A, B) Lycorine inhibited p65 and IκB phosphorylation. Prostate cells DU145 and PC3 cells were treated or untreated with TNF-α (20 ng/ml) for 30 min. After that, the cells were treated or untreated with Lycorine at final concentration as indicated for 12 h. Then cells were left untreated or exposed to Whole-cell extracts were prepared and analyzed by Western blot analysis using the indicated antibodies. (C) Lycorine inhibited p65 nuclear translocation in PC3 cells. PC3 cells were treated or untreated with TNF-α for 30 min, and then exposed to DMSO, lycorine or TPCA-1. Cytoplasmic and nuclear extracts representing equal numbers of cells were analyzed by Western blot with the indicated antibodies. (D) Lycorine inhibited p65 expression in PC3 cells via immunofluorescence. After treating with TNF-α for 30 min, PC3 cells were treated with DMSO, lycorine (5 µmol/L, 10 µmol/L) or TPCA-1. Immunostaining was performed with specific mouse anti-p65 antibody followed by Cy3-conjugated mouse anti-rabbit immunoglobulins (red) immediately after the treatment.