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. 2022 Aug 21;13(10):3151–3159. doi: 10.7150/jca.75597

Figure 3.

Figure 3

Lycorine induces G2/M phase cell cycle arrest in DU145 and PC3 cells. (A) We analyzed cell cycle distribution of DU145 Cells using PI staining. Cells were harvested for cell cycle distribution analysis using presidium iodide staining 24 hours after treatment with lycorine at the concentration as indicated. (B) We analyzed cell cycle distribution of PC3 Cells using PI staining. After treatment with lycorine for 24 h, the cells were harvested for cell cycle distribution analysis using presidium iodide staining. (C) The expression of cell cycle inhibitory protein p21 and cyclinD1 was detected by western blot in DU145 Cells. (D) The expression of cell cycle inhibitory protein p21 and cyclinD1 was detected by western blot in PC3 Cells.