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. 2022 Jul 27;14(8):1638. doi: 10.3390/v14081638

Figure 6.

Figure 6

Flowchart of common diagnostic methods for PRV infection. Two types of common methods are used to verify the PRV infection based on the PRV-specific antibodies and nucleic acids. Among them, serological approaches for the detection of PRV infection include the enzyme-linked immunosorbent assays (ELISA), indirect immunofluorescence assay (IFA), serum neutralization test (SNT), direct-immunofluorescence method (DFM) and blocking immunoperoxidase monolayer assay (b-IPMA). Molecular biology approaches include polymerase chain reaction (PCR), real-time PCR (RT-PCR), TaqMan real-time PCR (qPCR), nano PCR, droplet digital PCR (ddPCR), real-time recombinase-aided amplification (RT RAA), loop-mediated isothermal amplification (LAMP), real-time fluorescent detection (real-time RPA assay), duplex fluorescence melting curve analysis (FMCA), next-generation sequencing (NGS), probe-based fluorescence melting curve analysis (FMCA), real-time recombinase-aided amplification assay (RT RAA) and third-generation sequencing (TGS).