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. 2022 Jul 29;14(8):1679. doi: 10.3390/v14081679

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effect of silencing SG assembly components on TSWV replication. (A) Plant phenotype after VIGS-silencing Rbp47 and G3BP1-like genes, compared with GUS-silenced control plant. (B) qRT-PCR assay to quantify the silencing efficiency. ** p-value < 0.01. (C) Green fluorescence resulting from eGFP expression from a TSWV replicon in plants silenced on SG assembly components, and control plants. Plants were infiltrated with TSWV-eGFP replicon constructs 2.5-week post silencing and harvested at around 60 hpi. (D) Statistical analysis of GFP intensity in the silenced plants and control plants.