Figure 9.

The cloning and identification of capped leader sequences snatched from provided transcripts. (A) Schematic overview of GFP, nsGFP and LGFP constructs and the cloning strategy of TSWV mRNA leader sequences. (B) The relative distribution of snatched leader sequences collected during in vivo cap donor competition assays between GFP, nsGFP and LGFP transcripts, respectively, at standard condition (23 °C) and heat stress condition (37 °C). (C,D) Expression levels of GFP, nsGFP and LGFP transcripts as determined by qRT-PCR.