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. 2021 Aug 30;9(11):1298–1315. doi: 10.1158/2326-6066.CIR-21-0543

Figure 1.

Figure 1. The characteristics of the immune escaped tumor model. A, Strategy for establishing an immune escaped cancer cell line. B, FACS of H-2Kb–bound SIINFEKL, MHC-I, and PD-L1 (blue represents isotype). C, RT-PCR of SQ transcripts. D, Analysis of genomic SQ copy number. E, Animation of CpG methylation on SQ promoter. F, Quantification of methylated CpG sites on SQ promoter. G, DNA accessibility of SQ region evaluated by ATAC-seq. H, Quantification of DNA accessibility of SQ region. Two-tailed unpaired t tests were used to compare the two arms. Error bars represent mean ± SEM. * P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. NS, not significant.

The characteristics of the immune escaped tumor model. A, Strategy for establishing an immune escaped cancer cell line. B, FACS of H-2Kb–bound SIINFEKL, MHC-I, and PD-L1 (blue represents isotype). C, RT-PCR of SQ transcripts. D, Analysis of genomic SQ copy number. E, Animation of CpG methylation on SQ promoter. F, Quantification of methylated CpG sites on SQ promoter. G, DNA accessibility of SQ region evaluated by ATAC-seq. H, Quantification of DNA accessibility of SQ region. Two-tailed unpaired t tests were used to compare the two arms. Error bars represent mean ± SEM. * P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. NS, not significant.