Skip to main content
. 2021 Aug 30;9(11):1298–1315. doi: 10.1158/2326-6066.CIR-21-0543

Figure 2.

Figure 2. Atf7ip regulates SQ expression and presentation. A, Strategy of epigenetic CRISPR screen to identify epigenetic regulators of antigen expression and presentation. B, Volcano plot illustrating genes whose disruption enhanced (red) or inhibited (blue) presentation of SIINFEKL. C, Illustration of the top 10 candidates from B. D, Normalized read counts of Atf7ip sgRNAs in SIINFEKL 15% high and 15% low populations. E, RT-PCR of SQ transcripts. F, FACS of SIINFEKL presentation. G, Quantification of SIINFEKL presentation in F. H, Animation of CpG methylation on SQ promoter KP-IE2-C1 (the single clone of KP-IE2). I, Quantification of methylated CpG sites on SQ promoter. J, Representative MRI scans showing mouse lung tumors 4 weeks after transplant through tail vein injection in B6/J immunocompetent mice. K, Quantification of lung tumors volume in J (n = 3 mice/group), L, Survival curves for the immunocompromised nude mice (NU/NU) after being transplanted with control and Atf7ip-deficient KPIE2 cells (n = 3 mice/group). M, Survival curves for each group in the study (n = 9 mice/group). For survival data, results were plotted using Kaplan–Meier curve, and statistical significance was determined via a log-rank test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. NS, not significant.

Atf7ip regulates SQ expression and presentation. A, Strategy of epigenetic CRISPR screen to identify epigenetic regulators of antigen expression and presentation. B, Volcano plot illustrating genes whose disruption enhanced (red) or inhibited (blue) presentation of SIINFEKL. C, Illustration of the top 10 candidates from B. D, Normalized read counts of Atf7ip sgRNAs in SIINFEKL 15% high and 15% low populations. E, RT-PCR of SQ transcripts. F, FACS of SIINFEKL presentation. G, Quantification of SIINFEKL presentation in F. H, Animation of CpG methylation on SQ promoter KP-IE2-C1 (the single clone of KP-IE2). I, Quantification of methylated CpG sites on SQ promoter. J, Representative MRI scans showing mouse lung tumors 4 weeks after transplant through tail vein injection in B6/J immunocompetent mice. K, Quantification of lung tumors volume in J (n = 3 mice/group), L, Survival curves for the immunocompromised nude mice (NU/NU) after being transplanted with control and Atf7ip-deficient KPIE2 cells (n = 3 mice/group). M, Survival curves for each group in the study (n = 9 mice/group). For survival data, results were plotted using Kaplan–Meier curve, and statistical significance was determined via a log-rank test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. NS, not significant.